ATG16L1 Knockout cell line (Vero)

ATG16L1 Knockout cell line (Vero) Order

Catalog#

YKO-R058

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ATG16L1 Knockout cell line (Vero)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Gene Name

ATG16L1

Gene ID

103218104

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

Target region

Knockout region: Exon 1 (based on transcript ATG16L1-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATG16L1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Vero cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATG16L1 gene knockout ATG16L1 Knockout cell line (Vero) will be expanded and cryopreserved.

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